Primer

Part:BBa_K1456010:Design

Designed by: safa tapan   Group: iGEM14_ATOMS-Turkiye   (2014-08-13)


Glutathione Peroxidase (GPx) enzyme reverse primer with restriction site, two stop codon and 6X His


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 14
    Illegal SpeI site found at 22
    Illegal PstI site found at 2
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 14
    Illegal SpeI site found at 22
    Illegal PstI site found at 2
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 8
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 14
    Illegal SpeI site found at 22
    Illegal PstI site found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 14
    Illegal SpeI site found at 22
    Illegal PstI site found at 2
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

none


Source

none

References